Research council visitors: No-gag gag

No gag, no food.

New insights into retroviral Gag–Gag and Gag–membrane interactions

A critical aspect of viral replication is the assembly of virus particles, which are subsequently released as progeny virus. While a great deal of attention has been focused on better understanding this phase of the viral life cycle, many aspects of the molecular details remain poorly understood. This is certainly true for retroviruses, including that of the human immunodeficiency virus type 1 ...

DNA gag/adenovirus type 5 (Ad5) gag and Ad5 gag/Ad5 gag vaccines induce distinct T-cell response profiles.

Results from Merck's phase II adenovirus type 5 (Ad5) gag/pol/nef test-of-concept trial showed that the vaccine lacked efficacy against human immunodeficiency virus (HIV) infection in a high-risk population. Among the many questions to be explored following this outcome are whether (i) the Ad5 vaccine induced the quality of T-cell responses necessary for efficacy and (ii) the lack of efficacy i...

Role of RNA in facilitating Gag/Gag-Pol interaction.

We have examined the influence of RNA upon the interaction of Gag-Pol with Gag during human immunodeficiency virus type 1 (HIV-1) assembly. COS7 cells were transfected with protease-negative HIV-1 proviral DNA, and Gag/Gag-Pol complexes were detected by coimmunoprecipitation with anti-integrase. In COS7 cells, Gag/Gag-Pol is found almost entirely in pelletable, membrane-bound complexes. Exposur...

Severe Gag Reflex

Susceptibility to the gag reflex may render insertion of removable dentures very difficult. The use of intravenous sedation in such cases allows for the fabrication of dentures with decreased discomfort to the patient. When the completed dentures are inserted, however, discomfort may still occur as the effects of the gag reflex will again be felt. We report a case of an edentulous maxillary pat...